| Chris Pratt | | Emmet Brickowski / Rex Dangervest (voice) |
| Elizabeth Banks | | Wyldstyle / Lucy (voice) |
| Will Arnett | | Batman (voice) |
| Tiffany Haddish | | Queen Watevra Wa'Nabi (voice) |
| Stephanie Beatriz | | General Mayhem / Sweet Mayhem (voice) |
| Alison Brie | | Unikitty / Ultrakatty (voice) |
| Nick Offerman | | MetalBeard (voice) |
| Charlie Day | | Benny (voice) |
| Maya Rudolph | | Mom |
| Will Ferrell | | President Business / Dad |
| Jadon Sand | | Finn |
| Brooklynn Prince | | Bianca |
| Channing Tatum | | Superman (voice) |
| Jonah Hill | | Green Lantern (voice) |
| Richard Ayoade | | Ice Cream Cone (voice) |
| Ben Schwartz | | Banarnar (voice) |
| Noel Fielding | | Balthazar (voice) |
| Jason Momoa | | Aquaman (voice) |
| Cobie Smulders | | Wonder Woman (voice) |
| Ike Barinholtz | | Lex Luthor (voice) |
| Ralph Fiennes | | Alfred Pennyworth (voice) |
| Will Forte | | Abraham Lincoln (voice) |
| Jimmy O. Yang | | Enthusiastic Zebra (voice) |
| Jorma Taccone | | Larry Poppins (voice) |
| Bruce Willis | | Bruce Willis (voice) |
| Gary Payton | | Gary Payton (voice) |
| Sheryl Swoopes | | Sheryl Swoopes (voice) |
| Todd Hansen | | Gandalf / Swamp Creature (voice) |
| Doug Nicholas | | Surfer Dave / Chainsaw Dave / Purgatory Dave (voice) |
| Liam Knight | | Duplo / Heart / Star / Sewer Babies (voice) |
| Emmett Mitchell | | Duplo / Duplo Foreman / Duplo Wonder Woman (voice) |
| Sawyer D. Jones | | Duplo / Heart / Star (voice) |
| Graham Miller | | Duplo / Young Finn (voice) |
| Cora Miller | | Duplo / Star (voice) |
| Ollie Mitchell | | Duplo (voice) |
| Mike Mitchell | | Guard / Octopus / Harmony Town Citizen / Announcer / Sherry / Apocalypseburg Warrior (voice) |
| Christopher Miller | | Horse / Chocolate Bar / Plantimals / Paper Boy / Chad (voice) |
| Margot Rubin | | Susan / Mermaid / Harley Quinn / Wonder Woman Mini Doll / Panda (voice) |
| Emily Nordwind | | Cleopatra (voice) |
| Chris McKay | | Larry the Barista (voice) |