| Zach Braff | | Chicken Little (voice) |
| Garry Marshall | | Buck Cluck (voice) |
| Don Knotts | | Mayor Turkey Lurkey (voice) |
| Patrick Stewart | | Mr. Woolensworth (voice) |
| Amy Sedaris | | Foxy Loxy (voice) |
| Steve Zahn | | Runt of the Litter (voice) |
| Joan Cusack | | Abby Mallard (voice) |
| Wallace Shawn | | Principal Fetchit (voice) |
| Harry Shearer | | Dog Announcer (voice) |
| Fred Willard | | Melvin - Alien Dad (voice) |
| Catherine O'Hara | | Tina - Alien Mom (voice) |
| Patrick Warburton | | Alien Cop (voice) |
| Adam West | | Ace - Hollywood Chicken Little (voice) |
| Mark Walton | | Goosey Loosey (voice) |
| Mark Dindal | | Morkubine Porcupine / Coach (voice) |
| Dan Molina | | Fish Out of Water (voice) |
| Joe Whyte | | Rodriguez / Acorn Mascot / Umpire (voice) |
| Sean Elmore | | Kirby - Alien Kid (voice) |
| Evan Dunn | | Kirby - Alien Kid (voice) |
| Matthew Josten | | Kirby - Alien Kid (voice) |
| Kelly Hoover | | Mama Runt (voice) |
| Will Finn | | Hollywood Fish (voice) |
| Dara McGarry | | Hollywood Abby (voice) |
| Mark Kennedy | | Hollywood Runt (voice) |
| Pat Fraley | | Additional Voices (voice) |