 | Tom Cruise | | Narrator (voice) |
| James Arnold | | Self |
| Michael J. Bloomfield | | Self (Pilot, STS-97) |
| Robert D. Cabana | | Self (Commander, STS-88) |
| Leroy Chiao | | Self |
| Kenneth D. Cockrell | | Self |
| Robert L. Curbeam Jr. | | Self |
| Brian Duffy | | Self (Commander, STS-92) |
| Marc Garneau | | Self (Mission Specialist, STS-97) |
| Michael L. Gernhardt | | Self (Mission Specialist, STS-104) |
| Yuri Pavlovich Gidzenko | | Self (Soyuz Commander, Expedition One) |
| Umberto Guidoni | | Self |
| Chris Hadfield | | Self |
| Susan Helms | | Self (Expedition Two Flight Engineer) |
| Susan J. Helms | | Self |
| Charles Owen Hobaugh | | Self (Pilot, STS-104) |
| Marsha Ivins | | Self (Mission Specialist, STS-98) |
| Brent W. Jett Jr. | | Self (Commander, STS-97) |
| Tom D. Jones | | Self (Mission Specialist, STS-98) |
| Greg Jurls | | Self - Video Producer |
| Janet Lynn Kavandi | | Self (Mission Specialist, STS-104) |
| James M. Kelly | | Self (Pilot, STS-102) |
| Sergei Krikalev | | Self (Flight Engineer, Expedition One) |
| Steven W. Lindsey | | Self (Commander, STS-104) |
| Yuri Valentinovich Lonchakov | | Self (Mission Specialist, STS-100) |
| Michael E. López-Alegría | | Self |
| William Surles McArthur Jr. | | Self |
| Pamela Ann Melroy | | Self (Pilot, STS-92) |
| James H. Newman | | Self (Mission Specialist, STS-88) |
| Scott E. Parazynski | | Self |
| John L. Phillips | | Self (Mission Specialist, STS-100) |
| Mark L. Polnaksy | | Self |
| James F. Reilly | | Self |
| Paul William Richards | | Self |
| Kent V. Rominger | | Self (Commander, STS-100) |
| Jerry L. Ross | | Self (Mission Specialist, STS-88) |
| William M. Shepherd | | Self (Commander, Expedition One) |
| Joseph R. Tanner | | Self (Mission Specialist, STS-97) |
| Andrew S.W. Thomas | | Self |
| Yury Usachev | | Self (Expedition Two Commander) |
