 | Mike Myers | | Shrek (voice) |
| Eddie Murphy | | Donkey (voice) |
| Cameron Diaz | | Princess Fiona (voice) |
| Antonio Banderas | | Puss in Boots (voice) |
| Julie Andrews | | Queen (voice) |
| Jon Hamm | | Brogan (voice) |
| John Cleese | | King (voice) |
| Walt Dohrn | | Rumpelstiltskin / Priest / Krekraw Ogre (voice) |
| Jane Lynch | | Gretched (voice) |
| Craig Robinson | | Cookie (voice) |
| Lake Bell | | Patrol Witch / Wagon Witch #2 (voice) |
| Kathy Griffin | | Dancing Witch / Wagon Witch #1 (voice) |
| Mary Kay Place | | Guard Witch (voice) |
| Kristen Schaal | | Pumpkin Witch / Palace Witch (voice) |
| Meredith Vieira | | Broomsy Witch (voice) |
| Ryan Seacrest | | Father of Butter Pants (voice) |
| Cody Cameron | | Pinocchio / Three Pigs (voice) |
| Larry King | | Doris (voice) |
| Regis Philbin | | Mabel (voice) |
| Christopher Knights | | Blind Mice (voice) |
| Conrad Vernon | | Gingerbread Man (voice) |
| Aron Warner | | Wolf (voice) |
| Jasper Johannes Andrews | | Ogre Baby (voice) |
| Ollie Mitchell | | Ogre Baby (voice) |
| Miles Bakshi | | Ogre Baby / Villager Kid (voice) |
| Nina Zoe Bakshi | | Ogre Baby / Tourist Girl / Villager Girl (voice) |
| Billie Hayes | | Cackling Witch (voice) |
| Jeremy Hollingworth | | Villager Fan #1 (voice) |
| Brian Hopkins | | Villager Fan #3 / Ogre Gnimrach (voice) |
| Chris Miller | | Royal Messenger / Magic Mirror / Geppetto (voice) |
| Mike Mitchell | | Tour Guide / Camp Ogre / Ogre Naysayer / Baba Witch / Melty Witch / Witch Guard #2 / Butter Pants (voice) |
| James Ryan | | Villager Fan #2 (voice) |
