 | Jesse Eisenberg | | Blu (voice) |
| Anne Hathaway | | Jewel (voice) |
| Sofia Scarpa Saldanha | | Young Linda (voice) |
| Leslie Mann | | Linda (voice) |
| Wanda Sykes | | Chloe (The Goose) (voice) |
| Jane Lynch | | Alice (The Other Goose) (voice) |
| Kelly Keaton | | Bookstore Customer / Lady Tourist (voice) |
| Jamie Foxx | | Nico (voice) |
| Karen Disher | | Mother Bird (voice) |
| Jason Fricchione | | Truck Driver (voice) |
| Rodrigo Santoro | | Tulio / Soccer Announcer (voice) |
| Gracinha Leporace | | Dr. Barbosa (voice) |
| Jemaine Clement | | Nigel (voice) |
| Will.i.am | | Pedro (voice) (as will.i.am) |
| Jake T. Austin | | Fernando (voice) |
| Phil Miler | | Aviary Intern / Waiter (voice) |
| Bernardo De Paula | | Sylvio / Kipo (voice) |
| Carlos Saldanha | | Second Waiter (voice) |
| Renato D'Angelo | | Police Officer (voice) |
| Carlos Ponce | | Marcel (Lead Smuggler) (voice) |
| Jeffrey Garcia | | Tipa (Heavy Smuggler) / Bat (voice) |
| Davi Vieira | | Armando (Tall Smuggler) (voice) |
| Thomas F. Wilson | | Trapped Bird / Screaming Hang Glider (voice) |
| Cindy Slattery | | Neurotic Bird (voice) |
| Justine Warwick | | Scaredy Bird (voice) |
| George Lopez | | Rafael (voice) |
| Bebel Gilberto | | Eva (voice) |
| Judah Friedlander | | Tourist (voice) |
| Francisco Ramos | | Lead Marmoset (voice) |
| Tim Nordquist | | Other Hang Glider (voice) |
| Miriam Wallen | | Green Bird (voice) |
| Tracy Morgan | | Luiz (voice) |
| Carlos de Oliveira | | Gate Guard (voice) |
| Sergio Mendes | | Samba School Director (voice) |
| Ester Dean | | Boy in Gondola (voice) |
| Ubirajara de Castro | | Additional Voices (voice) |
