 | Tara Strong | | Princess Twilight Sparkle |
| Ashleigh Ball | | Applejack / Rainbow Dash |
| Andrea Libman | | Fluttershy / Pinkie Pie |
| Tabitha St. Germain | | Rarity / Princess Luna / Granny Smith / Muffins |
| Cathy Weseluck | | Spike the Dragon |
| Emily Blunt | | Tempest Shadow / Fizzlepop Berrytwist |
| Michael Peña | | Grubber |
| Liev Schreiber | | The Storm King |
| Taye Diggs | | Capper |
| Zoe Saldana | | Captain Celaeno |
| Kristin Chenoweth | | Princess Skystar |
| Max Martini | | Boyle |
| Uzo Aduba | | Queen Novo |
| Britt McKillip | | Princess Cadance |
| Peter New | | Big Mac / Klugetown Featured Voices |
| Mark Oliver | | First Mate Mullet |
| Nicole Oliver | | Princess Celestia / Lix Spittle / Klugetown Featured Voices |
| Adam Bengis | | Code Red |
| Sia | | Songbird Serenade |
| Sam Vincent | | Party Favor / Canterlot Featured Voices |
| Michelle Creber | | Applebloom |
| Andrew McNee | | Canterlot & Klugetown Featured Voices |
| Michael Dobson | | Bulk Biceps / Canterlot & Klugetown Featured Voices |
| Tegan Moss | | Canterlot Featured Voices |
| Sabrina Pitre | | Canterlot Featured Voices |
| Rhona Rees | | Canterlot Featured Voices |
| Richard Ian Cox | | Klugetown Featured Voices |
