 | Mike Myers | | Shrek (voice) |
| Eddie Murphy | | Donkey (voice) |
| Cameron Diaz | | Princess Fiona (voice) |
| Antonio Banderas | | Puss in Boots (voice) |
| Julie Andrews | | Queen (voice) |
| John Cleese | | King (voice) |
| Rupert Everett | | Prince Charming (voice) |
| Eric Idle | | Merlin (voice) |
| Justin Timberlake | | Artie (voice) |
| Susanne Blakeslee | | Evil Queen (voice) |
| Cody Cameron | | Pinocchio / Three Pigs (voice) |
| Larry King | | Doris (voice) |
| Christopher Knights | | Blind Mice / Heckler / Evil Tree #2 / Guard #2 (voice) |
| John Krasinski | | Lancelot (voice) |
| Ian McShane | | Captain Hook (voice) |
| Cheri Oteri | | Sleeping Beauty / Actress (voice) |
| Regis Philbin | | Mabel (voice) |
| Amy Poehler | | Snow White (voice) |
| Seth Rogen | | Ship Captain (voice) |
| Maya Rudolph | | Rapunzel (voice) |
| Amy Sedaris | | Cinderella (voice) |
| Conrad Vernon | | Gingerbread Man / Rumplestiltskin / Headless Horseman (voice) |
| Aron Warner | | Wolf (voice) |
| Jasper Johannes Andrews | | Ogre Baby (voice) |
| Guillaume Aretos | | Raul (voice) |
| Kelly Asbury | | Master of Ceremonies / Fiddlesworth (voice) |
| Zachary James Bernard | | Ogre Baby (voice) |
| Andrew Birch | | Evil Tree #1 (voice) |
| Sean Bishop | | Drivers Ed Instructor / Hall Monitor / Teacher (voice) |
| Kelly Cooney | | Cheerleader / Tiffany / Mother (voice) |
| Walt Dohrn | | Van Student / Xavier / Principal Pynchley / Nanny Dwarf / Evil Knight / Singing Villain (voice) |
| Dante James Hauser | | Ogre Baby (voice) |
| Jordan Alexander Hauser | | Ogre Baby (voice) |
| Tom Kane | | Guard #1 (voice) |
| Tom McGrath | | Gary (voice) |
| Chris Miller | | Puppet Master / Announcer / Mascot / Singing Villain (voice) |
| Latifa Ouaou | | Cheerleader / Guinevere / Woman (voice) |
| Alina Phelan | | Cheerleader (voice) |
| David P. Smith | | Waiter / Evil Dwarf (voice) |
| Mark Valley | | Cyclops (voice) |
