 | Tom Hanks | | Woody (voice) |
| Tim Allen | | Buzz Lightyear (voice) |
| Joan Cusack | | Jessie the Yodeling Cowgirl (voice) |
| Kelsey Grammer | | Stinky Pete the Prospector (voice) |
| Don Rickles | | Mr. Potato Head (voice) |
| Jim Varney | | Slinky Dog (voice) |
| Wallace Shawn | | Rex the Green Dinosaur (voice) |
| John Ratzenberger | | Hamm the Piggy Bank (voice) |
| Annie Potts | | Bo Peep (voice) |
| Wayne Knight | | Al the Toy Collector (voice) |
| John Morris | | Andy (voice) |
| Laurie Metcalf | | Andy's Mom (voice) |
| Estelle Harris | | Mrs. Potato Head (voice) |
| R. Lee Ermey | | Sarge (voice) |
| Jodi Benson | | Tour Guide Barbie / Barbie on Backpack (voice) |
| Jonathan Harris | | The Cleaner (voice) |
| Joe Ranft | | Wheezy / Heimlich (voice) |
| Andrew Stanton | | Emperor Zurg (voice) |
| Jeff Pidgeon | | Green Aliens / Mr. Spell (voice) |
| Jack Angel | | Rocky Gibraltar / Shark (voice) |
| Bob Bergen | | Green Army Men (voice) |
| Sheryl Bernstein | | (voice) |
| Rodger Bumpass | | Clerk (voice) |
| Corey Burton | | Woody's Roundup Announcer (voice) |
| Rachel Davey | | (voice) |
| Debi Derryberry | | Green Aliens / Amy (voice) |
| Jessica Evans | | Woman #2 (voice) |
| Bill Farmer | | Man #1 (voice) |
| Pat Fraley | | Toy Store Buzz Lightyears (voice) |
| Jess Harnell | | Man #2 (voice) |
| John Lasseter | | Blue Rock 'Em Sock 'Em Robot (voice) |
| Nicolette Little | | Little Girl at Yard Sale (voice) |
| Sherry Lynn | | Barbie Girl (voice) |
| Mickie McGowan | | Mom at yard sale (voice) |
| Andi Peters | | Additional voice (voice) |
| Phil Proctor | | Sign-off voice / Airline rep / Mr. Konishi (voice) |
| Jan Rabson | | Japanese Businessman (voice) |
| Carly Schroeder | | (voice) |
| Madylin Sweeten | | Woman #1 (voice) |
