| Fred Rogers | | Self |
| Margaret Whitmer | | Self - Producer, Mister Rogers' Neighborhood |
| Tom Junod | | Self - Journalist / Friend |
| Joanne Rogers | | Self - Fred's Wife |
| Josie Carey | | Self |
| Jim Rogers | | Self - Fred's Son |
| Max King | | Self - Biographer |
| Hedda Sharapan | | Self - Child Development Advisor |
| Betty Seamans | | Self - 'Mrs. McFeely' |
| Junlei Li | | Self - Co-Director, Fred Rogers Center |
| Joe Negri | | Self - 'Handyman Negri' |
| David Newell | | Self - 'Mr. McFeely' |
| Bill Isler | | Self - Colleague / Friend |
| John Rogers | | Self - Fred's Son |
| Elaine Crozier | | Self - Fred's Sister |
| George Wirth | | Self - Pastor / Friend |
| Ralph Ellison | | Self - Author / Member of The Carnegie Commission |
| David Bianculli | | Self - Television Critic |
| John O. Pastore | | Self - Senator |
| Yo-Yo Ma | | Self - Friend |
| François Scarborough Clemmons | | Self - 'Officer Clemmons' |
| Nick Tallo | | Self - Floor Manager, Mister Rogers' Neighborhood |
| Lorin Hollander | | Self - Concert Pianist |
| Susan Stamberg | | Self - Journalist |
| Howard Erlanger | | Self - Jeff Erlanger's Dad |
| Pam Erlanger | | Self - Jeff Erlanger's Mom |
| Jeff Erlanger | | Self |
| Betty Aberlin | | Lady Aberlin |
| McColm Cephas Jr. | | Self |
| Bill Clinton | | Self |
| Hillary Clinton | | Self |
| Johnny Costa | | Self |
| Kailyn Davis | | Self |
| Deisy Garcia | | Self |
| Mason Gloria | | Self |
| Al Gore | | Self |
| Lincoln Howell | | Self |
| Lyndon B. Johnson | | Self |
| Michael Keaton | | Self |
| Robert F. Kennedy | | Self |